Microfluidics: Chemotaxis and ADVANTAGES
it is known microfluidics nowadays is revolutionizing the way the motile behaviour of cells are studied.
It has enabled observations at high spatial and temporal resolution is carefully controlled microenvironments.
Taking into account both of the parts, chemotaxis and microfluidics, there are some advantages when using them two together:
– Presents experimental challenges that are not observed on chemotaxis studies of surface-adherent cells.
– Generate steady, arbitrarily shaped chemical gradients.
– Unique control over the chemoattractant gradient and the migration environment of cells.
– Provide spatial and temporal control over a reproducible chemical environment.
– Enables building well defined and stable chemical gradients at cellular length scales.
– Provide a platform for quantifying cellular responses at cellular and molecular levels.
Microfluidics Application: Chemotaxis
When an organism moves in response to a chemical stimulus it is said that Chemotaxis has happened.
Due to some chemicals that are in the environment the organisms, such as Somatic cells, bacteria, single-cell or multicellular organisms, direct their movements.
When the chemotaxis movements occur toward a higher concentration of the chemical, it is said that is a positive movement.
On the contrary, when the movement is done in the opposite direction, toward the lowest concentration, it is said that is a negative movement.
In addition, if it is nondirectional or randomly directed, the chemotaxis will be called Chemokinesis.
Chemotaxis is done with two independent chemoattractants.
Even if they are independent, we can say that they are interrelated processes-motility and directionality and both of them are regulated by extracellular stimuli.