Microfluidics in the Catalogue
MicroLIQUID integrated in the new BIOBASQUE Catalog, where all the information of the biotech companies of the Basque Region is available.
Here you can get the full information about the companies:
The BASQUE BIOCLUSTER is a non-profit association constituted in 2010, and represents the idea of enhancing the biosciences sector in the Basque Country. The coordination, the collaboration with all the stakeholders in the sphere of biosciences, promoting business cooperation is the basis for the competitive development of its companies and their internationalisation, and is greatly contributing to the development and positioning of the Basque bio sector.
Today the Basque Biocluster has 30 partner businesses, 58 including their subsidiaries, which in 2014 had a turnover of €268 million, 78.7% (€211 million) of which came from the export of their products and services to international markets. As a whole, the companies in the association contribute to the maintenance of 1,632 jobs.
Industry based on biosciences is characterised by the close relationship between research, innovation and competitiveness, and largely rests on the appearance of a new type of companies whose objective is to exploit advanced technologies related to the sciences of life, in order to respond to myriad needs in various industrial spheres.
In today’s catalog, more than 80 entities are dedicated to biotechnology research, of which 51 had biotechnology as their main or exclusive activity, assigning more than half of their internal expenses to biotechnology R&D. Of these 80 entities, 70 are biotechnology companies.
The bio sector employs 1,183 people full time, or in other words, 6.4% of total R&D personnel in the Basque Country, with the outstanding presence of women, who represent more than 60% of all people working full time in biotechnology.
Research personnel, for their part, stand at 903 people, 76% of the total.
Microfluidics Application: Point of Care Devices
Our aim is to provide portable diagnostic tools to ensure rapid, affordable and simple analysis in many scenarios of our society (hospitals, airports, doctor’s practice, roadside police controls, natural environment etc).
However, conventional analytical methods often require a large volume of sample and complicated time-consuming protocols.
The more portable ones are based on slow immunochromatographic strips or low-sensitivity electrochemical detection systems, whereas desktop systems are sensitive and semi-automatic but bulky and heavy.
microLIQUID tries to improve quality of life and medical services through the development of quick diagnostic devices that will carry out sample preparation and detection reducing the incidence of current society threats.
Our idea is to create intelligent and portable systems across many sectors for efficient treatment(environment monitoring, health, food , veterinary), by integrating cost-efficiently manufactured Lab-on-a-Chips.
Microfluidics Manufacturing: Micromilling
Micromilling in Microfluidics:Using small cutting tools to create microfluidic architectures.
It is a mechanical method, in which we use small cutting tools to remove the materials from a specific part of the microfluidic architecture.
PDMS structure cannot be obtained directly by this method. The replica of the design can be machined and the mould(are reusable) can be casted in order to get PDMS devices.
Technique: A computer is used to control the position of the tool and the cutting of the structures.The milling time,depends on the milling structure, and can vary from minutes to hours .
The advantage of this technique is that it does not affect the polymeric material by UV radiation or heating, but it creates stress near the cut structures that can be avoided by heating and slow cooling.
Microfluidics: Liquid handling
The aim of this document is to scratch the surface of microfluidics, trying to describe the most significant phenomena at this scale.
The first goal of microfluidics is to take advantage of the benefits of scaling down: better control, lower cost, faster results and more. These benefits are especially relevant for biological reactions.
The effects that become dominant in microfluidics include laminar flow, diffusion, fluidic resistance, surface area to volume ratio, and surface tension. As the magnitude of these physical effects is different to the ones experienced at the macroscopic scale, fluid integrated microdevices must be designed from first principles and not simply by miniaturizing macroscopic devices.
Liquid flow in the microdomain belongs to the regime of viscous dominated flow. There is a fundamental change in hydrodynamics that occurs here, which significantly affects microfluidic operations like mixing. This barrier occurs when the Reynolds number is of the order of unity. At these scales, viscous forces dominate over inertial forces, turbulence is nonexistent, surface tension can be a powerful force, diffusion becomes the basic method for mixing, and evaporation acts quickly on exposed liquid surfaces. At low Reynolds numbers, fluid dynamics is dominated by viscous drag rather than by inertia and this is why devices that rely on inertial effects for their operation will no longer work.
One consequence of laminar flow is that two or more streams flowing in contact with each other will not mix except by diffusion. Diffusion is the process, by which a concentrated group of particles in a volume will, by Brownian motion, spread out over time so that the average concentration of particles throughout the volume is constant.
As diffusion times can be short at the microscale, microchannels can be used to create concentration gradients having complex profiles. Mixing schemes at the microscale must find ways to maximize the interfaces between solutions to allow diffusion to act quickly.
Surface area to volume ratio is another factor that becomes important at the microscale. The inverse characteristic length scaling of the surface-area-to-volume ratio implies that heat and mass transfer into or out of a chip can be enhanced as the dimensions of the device are reduced.
When working at the micro scale, another element as the surface tension forces become significant. Surface tension is the result of cohesion between liquid molecules at the interfaces. The surface free energy of a liquid is a measure of how much tension its surface contains. The path a fluid will travel through a capillary is directly related to the water’s surface free energy and inversely related to the radius of the capillary.
When microchannels with dimensions on the order of microns are used, the lengths liquids travel based only on capillary forces are significant. Surface energies have been widely exploited in microfluidics as pumping systems.
Microfluidics deals with the behaviour, precise control and manipulation of fluids that are geometrically constrained to a small (typically sub-millimetre) scale. This kind of research and work involves the usage of different technologies, components and materials, witch are key factors in microfluidic area.
Usually, micro means one of the following features:
Microfluidics is a multidisciplinary field intersecting engineering, physics, chemistry, microtechnology and biotechnology, with practical applications to the design of systems in which such small volumes of fluids will be used. Microfluidic area emerged in the beginning of the 1980s and is used in the development of inkjet printheads, DNA chips,lab on a chip technology, micro-propulsion, and micro-thermal technologies.
In this field microLIQUID develops and produces from the simplest microfluidic chip to complex microfluidic devices.
Our manufacture process allows us to integrate different designs and devices in a wafer, reducing time and cost of manufacturing.
microLIQUID offers standard microfluidic products ( microfluidic chips and encapsulate) and develop customized microfluidic structures and chip holders (connectors).
Microfluidic Prototyping: Molds for PDMS Microfluidic Chips
Microfluidic Prototyping systems
In microLIQUID, we fabricate the microfluidic structures according to the customer specific requirements.
With no minimum quantities requirements, we start the fabrication from 1 unit at affordable prices.
Our in-house facilities allows us to use different fabrication methods and/or materials.
The microfluidic channel geometries may vary with high aspect ratio, curved channels, high precision height or length and no depth-width constraints.
Experience:We’ve been developing our microfluidic expertise in the last years through several projects as: Microprobe flexible-semiflexible, Combining delivery and electrical signalling, qPCR Lab on a Chip, Electrolysis pump, Embedded microcantilevers, SU8 free standing structures embedded in microchannels for microfluidic control
Microfluidics Manufacturing: Photolitography technique
Photolitography is manufacturing process commonly used in microfluidics.
When it comes to challenging microfluidic arquitectures such as interdigitated electrodes(<10µ), high aspect ratio channels and small features this is a much more precise technique for the fabrication. It uses UV light to transfer a geometric pattern from a Cromium (<10µ) or acetate(>10µ) photomask with very high dimensional resolution
This manufacturing process can transfer these patterned structures to a wafer made of Silicon, Quartz, Glass, Polymers or even Metals.
The alignment of the photomask is a crucial process and is done with a mask aligner for accurate results.
The photolitography, when using the light, only allows to create rectangular or square channels, as the “attack” comes from the upper side and is straight(90º).
Also is used for manufacturing of molds for PDMS casting or hot embossing.
Microfluidic Chips: Injection Molding
Injection Molding in microfluidics is the last process of the development and launch of a microfluidic product.
Once the whole process of a microfluidic product workflow is done, and the product has interest for the market, the prototyping system has to be abandoned and the manufacturing process changes.
Due to more need of microfluidic chips manufacturing and once the material of the microfluidic architecture is decided, the technique to obtain fast and cost effective pieces is the INJECTION MOLDING.
Injection molding is basically the process to melt a thermoplastic under certain conditions and then, pushed within the cavities of a mold(injected), where it is cooled to a temperature. Once this temperature is reached, the pieces can be removed without deformation.
Once the mold is designed and done(is the main investment at this moment), the manufacturing process is replicable and the production batches can be enlarged and costs reduced. Time and cost shavings make this technique the best one to manufacture large series of microfluidic cartridges.
The molds can have only one cavity or several cavities(which allows to have more production per injection slot but costs are higher) and the polish process is a key element to obtain higher quality microfluidic cartridges.
Once you have the mold totally refined, the company is prepared for mass production of the microfluidic pieces.
Cyclic olefin polymer COP is an amorphous polyolefin with a cyclic structure in the main chain.
The following figure shows the polymerization scheme and polymer structures:
According to it, COP is polymerized by Ring Opening Metathesis Polymerization of norbornene derivatives, followed by hydrogenation of double bonds that provides more stability in terms of heat and weather resistance.
Compared to other thermoplastics such as PP, PC, PS and LDPE, COP provides significantly improved moisture and vapor barrier properties
COP is a glass-like & UV transparent polymer, which exhibits excellent optical properties and performances which enable to get a higher optical signal quality for small complex parts, increase resolution and lower detection limits in fluorescence spectroscopy, as well as to get a high dimensional stability under a harsh and humid environment. This material exhibits high transparency (92% light transmittance).
COP offers extremely low fluorescence across the excitation/emission spectrum and has been proven to increase resolution and lower detection limits in fluorescence spectroscopy for in vitro and in vivo imaging.
COP absorbs virtually no moisture and shows no dimensional changes even under conditions of high temperature and humidity. Thanks to its low water absorption, the refractive index of COP remains constant after exposure to humid environments.